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DNA replication in Eukaryotes by VEX

  DNA replication in eukaryotes This process occurs in three stages.    1. Initiation     2. Elongation   3. Termination   Initiation •       DNA replicatio n is initiated from the origins of replication ( Ori ), and eukaryotic nuclear DNA has multiple ori's. •      Initiation of replication first involves the formation of the pre-replicative complex ( pre-RC ). •      In yeast ( eukaryotic ) nuclear DNA replication , it starts with the binding of the origin recognition complex ( ORC ) to the ori region which is called autonomously replicating sequence ( ARS ). •  As cells enter the G1 phase of the cell cycle, ATP bound ORC bound to the ARS   recruits ATP-bound Cdc6 & two copies of the Mcm2-7 helicase bound to a second helicase loading protein ( Cdt1 ) to form a protein complex called pre-RC complex. •    This assembly of proteins tri...
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DNA replication in Prokaryotes complete notes

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  DNA replication in prokaryotes   • The circular chromosomal DNA of E.coli is replicated bidirectionally from a single origin of replication, oriC .   •  This mode of replication is also known as θ -replication. •  This process occurs in three stages.    Initiation Elongation Termination Initiation This involves recognition of the position on a DNA molecule where replication will begin.   Elongation This involves the events occuring at the replication fork , where the parent DNA strands are copied .   Termination This occurs when the parent DNA molecule has been completely replicated.   Click here for free lectures on Biology - ViologiaEXtrema Initiation of DNA replication in E.coli ·        DnaA protein initiates replication by binding to the oriC . ·        Binding of DnaA facilities the melting of duplex DNA. ·        Further melting ...
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